英语翻译Analytical determinationsBiomass concentration was determined by optical densitymeasurements at 600 nm.Vanillate,vanillin and ferulateconcentrations were determined by HPLC 1100,Hewlett Packard(Palo Alto,CA) provided with a Vydac 201TP54

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英语翻译Analytical determinationsBiomass concentration was determined by optical densitymeasurements at 600 nm.Vanillate,vanillin and ferulateconcentrations were determined by HPLC 1100,Hewlett Packard(Palo Alto,CA) provided with a Vydac 201TP54

英语翻译Analytical determinationsBiomass concentration was determined by optical densitymeasurements at 600 nm.Vanillate,vanillin and ferulateconcentrations were determined by HPLC 1100,Hewlett Packard(Palo Alto,CA) provided with a Vydac 201TP54
英语翻译
Analytical determinations
Biomass concentration was determined by optical density
measurements at 600 nm.Vanillate,vanillin and ferulate
concentrations were determined by HPLC 1100,Hewlett Packard
(Palo Alto,CA) provided with a Vydac 201TP54 C18 reverse-
phase column (Hesperia,CA),maintained at 35ºC,using an UV/
Vis detector,Hewlett Packard (Palo Alto,CA),set at λ = 254 nm.
The mobile phase,used at 0.5 mL/min flow rate,consisted of
40% methanol and 60% of a solution prepared adding 6.8 g/L
KH2PO4 in water and adjusting the pH with H3PO4 up to 2.5.
Data acquisition and processing were controlled by the Rev.
A.09.03 ChemStation Software,Agilent Technologies (Palo Alto,
CA).
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英语翻译Analytical determinationsBiomass concentration was determined by optical densitymeasurements at 600 nm.Vanillate,vanillin and ferulateconcentrations were determined by HPLC 1100,Hewlett Packard(Palo Alto,CA) provided with a Vydac 201TP54
Analytical determinations
解析的测定
Biomass concentration was determined by optical density measurements at 600 nm.
生物量的浓度是靠600纳米的光学密度来决定的.
Vanillate,vanillin and ferulate concentrations were determined by HPLC 1100,
羟基,香草醛和阿魏酸的浓度靠高性能液体色谱1100来判断
Hewlett Packard (Palo Alto,CA) provided with a Vydac 201TP54 C18 reverse-phase column (Hesperia,CA),maintained at 35oC,using an UV/
Vis detector,
Hewlett Packard 提供一个Vydac 201TP54 C18 反相高压液相层析,保持在35摄氏度,用一个紫外线探测仪.
Hewlett Packard (Palo Alto,CA),set at λ = 254 nm.
把波长设置为254纳米
The mobile phase,used at 0.5 mL/min flow rate,consisted of 40% methanol and 60% of a solution prepared adding 6.8 g/L KH2PO4 in water and adjusting the pH with H3PO4 up to 2.5.
流动相,每分钟0.5毫升,用40%的甲醇和60%的溶液,包含把6.8g/l的磷酸二氢钾加入水中,再把磷酸的酸碱度升到2.5
Data acquisition and processing were controlled by the Rev.A.09.03 ChemStation Software,Agilent Technologies (Palo Alto,
CA).
以上数据的获得,由Rev.A 09.03 ChemStation Software Agilent Technologies操作.

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分析测定
生物量取决于光密度浓度
测量在600海里。Vanillate、香草醛、亭
摘要采用HPLC测定浓度1100,惠普公司
(帕洛阿尔托,CA)提供Vydac 201TP54 C18反-
阶段栏(Hesperia、钙),维持在使用一个35ºC紫外/
能见度探测器,惠普公司(帕洛阿尔托,CA)定在λ= 2.54海里。
用于流动相的...

全部展开

分析测定
生物量取决于光密度浓度
测量在600海里。Vanillate、香草醛、亭
摘要采用HPLC测定浓度1100,惠普公司
(帕洛阿尔托,CA)提供Vydac 201TP54 C18反-
阶段栏(Hesperia、钙),维持在使用一个35ºC紫外/
能见度探测器,惠普公司(帕洛阿尔托,CA)定在λ= 2.54海里。
用于流动相的0.5毫升/分钟流量、由
40%甲醇和60%的解决方案准备增加6.8总帐
KH2PO4水和调节pH值,与H3PO4 2.5。
数据采集与处理受牧师。
A.09.03 ChemStation软件,安捷伦(帕洛阿尔托,
CA)。

收起

分析测定
生物量浓度测定光密度
尺寸在600纳米。香草,香草醛,阿魏酸
浓度测定高效液相色谱法1100年,惠普
(帕洛阿尔托,加利福尼亚州)和C18的一个Vydac 201TP54提供反向
相柱(Hesperia的,加利福尼亚州),维持在35℃,使用紫外/
可见检测器,惠普(帕洛阿尔托,加利福尼亚州),为λ= 254纳米。
流动...

全部展开

分析测定
生物量浓度测定光密度
尺寸在600纳米。香草,香草醛,阿魏酸
浓度测定高效液相色谱法1100年,惠普
(帕洛阿尔托,加利福尼亚州)和C18的一个Vydac 201TP54提供反向
相柱(Hesperia的,加利福尼亚州),维持在35℃,使用紫外/
可见检测器,惠普(帕洛阿尔托,加利福尼亚州),为λ= 254纳米。
流动相:0.5毫升/分钟流量使用,包括
40%甲醇和60%的解决方案准备加入6.8克/升
在水和磷酸二氢钾用磷酸调节pH值至2.5。
数据采集和处理是控制牧师
A.09.03化学工作站软件,(Palo Alto的安捷伦科技,
加利福尼亚州)。

收起